Molecular imaging
FRET microscopy and spectroscopy
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Author
Contributions
- Periasamy, Ammasi - Contributor
- Day, Richard N - Contributor
- American Physiological Society (1887- ) - Contributor
Publication
2005 - Oxford University Press, Oxford, New York (State)
Language
English
Word Count
78,000 words, Guess
Page Count
312 pages
Identifiers
- Open LibraryOL17186889M
- ISBN-100195177207
- OCLC Control Number57406048
- Library of Congress Control Number2004061702
- Goodreads5007619
and 1 more
- LibraryThing7101544
Classifications
- LCCQH212.F55 M64 2005
Description
Covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions.
Subjects
Topics
Series Statement
- The American Physiological Society methods in physiology series
Other Editions
- Molecular imaging
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